Simple and reliable method to precipitate proteins from bacterial culture supernatant.

نویسندگان

  • Randolph B Caldwell
  • Claus T Lattemann
چکیده

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 70 1  شماره 

صفحات  -

تاریخ انتشار 2004