Simple and reliable method to precipitate proteins from bacterial culture supernatant.
نویسندگان
چکیده
A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 70 1 شماره
صفحات -
تاریخ انتشار 2004